By Marvin C. McMaster(auth.)
Up to date and accelerated, the vintage consultant to GC/MS is helping chromatographers quick learn how to use this system for studying and making a choice on compounds. After explaining the basics, it discusses optimizing, tuning, utilizing, and retaining GC/MS apparatus; explores advances in miniaturized and field-portable GC/MS structures and microfluidic elements; and extra. entire with a CD-ROM, it covers functions within the environmental laboratory and in forensics, toxicology, and area technological know-how. this can be the foremost source for pros in these fields and for students.Content:
Chapter 1 advent (pages 1–20):
Chapter 2 pattern guidance and creation (pages 21–28):
Chapter three The fuel Chromatograph (pages 29–36):
Chapter four The Mass Spectrometer (pages 37–46):
Chapter five Getting began in GC/MS (pages 47–55):
Chapter 6 Chromatographic tools improvement (pages 57–65):
Chapter 7 Mass Spectrometer Setup and Operation (pages 67–76):
Chapter eight info Processing and community Interfacing (pages 77–83):
Chapter nine approach upkeep and Troubleshooting (pages 85–92):
Chapter 10 GC/MS within the Environmental Laboratory (pages 93–107):
Chapter eleven GC/MS in Forensics, Toxicology, and area technological know-how (pages 109–112):
Chapter 12 An advent to Structural Interpretation (pages 113–118):
Chapter thirteen Ion catch GC/MS platforms (pages 119–125):
Chapter 14 different GC/MS structures (pages 127–135):
Chapter 15 An advent to LC/MS (pages 137–143):
Chapter sixteen Innovation in GC/MS (pages 145–149):
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Extra info for GC/MS: A Practical User's Guide, Second Edition
When your sample is ready for injection, turn on the gas chromatograph and set the equilibration temperature for the injection. This is usually around 50À120 C, but we will use 50 C for this injection. We can now program the column oven for our run. We will set a hold at 50 C for 1 min, and then ramp from 50 to 320 C at 30 C/min. 50 GETTING STARTED IN GC/MS Since we decided we would use SCAN mode for our run, we need to select a scan range of 50À550 amu. We will work above 32 amu to avoid contamination from dissolved air.
They may require preparation of volatile derivatives or other treatment to aid in detection. 1 DIRECT SAMPLE INJECTION INTO THE MASS SPECTROMETER Many GC/Ms systems have a port for a direct insertion probe (DIP) on which a sample may be inserted directly into the mass spectrometer source for ionization and analysis. The sample can be introduced as a drop of liquid, a solid, as a film dissolved in a volatile solvent, or as an emulsion or suspension in an activating compound for fast atom bombardment (FAB).
Purge-and-trap apparatus is used to remove organics by gas sparging out of solution, trap them in a retention column, and then inject these compounds onto the GC column by reversing the gas flow and heating the trapping column. Volatile organics may be dissolved and injected in a carrier GC/MS: A Practical User’s Guide, Second Edition. By Marvin C. McMaster Copyright # 2008 John Wiley & Sons, Inc. 21 22 SAMPLE PREPARATION AND INTRODUCTION solvent or extracted from an aqueous mixture with a carrier solvent, dried to remove moisture, and injected.