Gas Chromatography and Lipids: A Practical Guide by William W. Christie

By William W. Christie

Gasoline chromatography was once first built through lipid analysts and lipid analysts were on the vanguard within the improvement of the process.

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C. THE PREPARATION OF METHYL AND OTHER ESTERS OF FATTY ACIDS The preparation of the methyl ester derivatives of fatty acids must be by far the commonest chemical reaction performed by lipid analysts, yet it is often poorly understood; the topic has been comprehensively reviewed [160,205,839]. There is no need to hydrolyse lipids to obtain the free fatty acids before preparing the esters as most lipids can be transesterified directly. No single reagent will suffice, however, and one must be chosen that best fits the circumstances.

G. ) and a few other volatile substances do not give a substantial signal. The principle of the detector is that ions are generated by combustion of the organic compounds as they emerge from the column in a diffusion flame of hydrogen and air. The carrier gas from the column may be premixed with hydrogen, although with WCOT columns, it is usual to position the outlet of the column at the orifice of the combustion jet in a chamber through which an excess of air is passed. The collector electrode is cylindrical and is placed just above the flame, and the ion current is measured by establishing a potential between the collector and the jet tip.

The instrument manufacturer's instructions should be followed closely to optimise these, although there is usually some leeway, as it is not easy to give general guidelines for instruments of different makes. W. J. Barnes & Assoc. (Oily Press) The nature of the response in relation to specific lipids is discussed in subsequent chapters. 2. Electron-capture detectors In the electron-capture detector, a radioactive source is used to bombard the carrier gas with β particles as it passes through an ionisation chamber.

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