By R. Ian Freshney(auth.)
This is the 6th variation of the best textual content within the simple technique of cellphone tradition, around the globe. carefully revised, it beneficial properties updates on really good suggestions in stem telephone learn and tissue engineering; updates on molecular hybridization, somatic telephone fusion, hybridomas, and DNA move; new sections on vitrification and Organotypic tradition, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. it really is written for graduate scholars, learn and scientific scientists, and technicians and laboratory managers in mobilephone and molecular biology labs and genetics labs.
PowerPoint slides of the figures in addition to different supplementary fabrics can be found at a significant other website: www.wiley.com/go/freshney/cellculture
Chapter 1 creation (pages 1–10):
Chapter 2 Biology of Cultured Cells (pages 11–23):
Chapter three Laboratory layout, structure, and gear (pages 25–36):
Chapter four apparatus and fabrics (pages 37–56):
Chapter five Aseptic strategy (pages 57–70):
Chapter 6 protection, Bioethics, and Validation (pages 71–88):
Chapter 7 tradition Vessels and Substrates (pages 89–98):
Chapter eight outlined Media and supplementations (pages 99–114):
Chapter nine Serum?Free Media (pages 115–132):
Chapter 10 education and Sterilization (pages 133–162):
Chapter eleven fundamental tradition (pages 163–186):
Chapter 12 tradition and phone traces (pages 187–206):
Chapter thirteen Cloning and choice (pages 207–225):
Chapter 14 mobilephone Separation (pages 227–237):
Chapter 15 Characterization (pages 239–268):
Chapter sixteen Differentiation (pages 269–278):
Chapter 17 Transformation and Immortalization (pages 279–297):
Chapter 18 infection (pages 299–315):
Chapter 19 Cryopreservation (pages 317–334):
Chapter 20 Quantitation (pages 335–364):
Chapter 21 Cytotoxicity (pages 365–381):
Chapter 22 really expert Cells (pages 383–432):
Chapter 23 Stem Cells, Germ Cells, and Amniocytes (pages 433–462):
Chapter 24 tradition of Tumor Cells (pages 463–479):
Chapter 25 Three?Dimensional tradition (pages 481–495):
Chapter 26 Scale?Up and Automation (pages 497–515):
Chapter 27 really good thoughts (pages 517–532):
Chapter 28 education courses (pages 533–568):
Chapter 29 challenge fixing (pages 569–591):
Chapter 30 In end (page 593):
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Additional info for Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition
10 min after removal of trypsin and ready for dispersing and counting. Phase contrast; 20x objective. Plate 5. Subculture by Trypsinization. 5 mm (a) HeLa Clones. HeLa-S3 cloned by dilution cloning and stained with Giemsa after 3 weeks. Scale bar 5 mm. (b) NRK Clone. Small clone of NRK cells, cloned by dilution cloning. Phase contrast, 4x objective. (c) Breast Carcinoma, JUW, Cloned on Plastic. Microphotograph of secondary culture from human breast carcinoma, 4000 cells/cm2, growing on plastic.
Tissue culture became more widely used at this time because of the introduction of antibiotics, which facilitated long-term cell line propagation, although many people were already warning against continuous use and the associated risk of harboring cryptic, or antibiotic-resistant, contaminations [Parker, 1961]. 6). Throughout this book the term tissue culture is used as a generic term to include organ culture and cell culture. The term organ culture will always imply a three-dimensional culture of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo.
Y. ) (e) View of Blood Vessels in Human Umbilical Cord. 18A). Black arrows, umbilical arteries. White arrow, umbilical vein with Luer adaptor inserted. (Courtesy of Charlotte Lawson; see also Fig. ) Plate 8. Finite Cell Lines and Cannulation of Human Umbilical Cord. (a) HeLa-S3. Human cervical carcinoma. Phase contrast. 40x objective. (c) MOG-G-UVW. Human glioma. Giemsa stained. 10x objective. (b) A549. Human lung adenocarcinoma. Giemsa stained. 10x objective. (d) Caco-2. Human colorectal carcinoma showing dome formation.