Ciba Foundation Symposium 189 - Cell Adhesion and Human

Specialists of their respective fields current papers involved in the diversity of human illnesses because of faulty or irregular functioning of telephone adhesion molecules. Discusses new healing ways to those maladies.

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A ckno wledgements We would like to thank Doug Larigan, Joe Levine, Lucy Foppiani, Rongshen Hsiao and John Duker for oligonucleotides and sequencing. We are also grateful t o Dr Greg Pirozzi and Gwen Wong for helpful discussions. References Abbondanzo SJ, Gadi 1, Stewart CL 1993 Derivation of embryonic stem cell lines. Methods Enzymol 225903-823 Becker-AndrC M, Van Huijsduijnen RH, Losberger C , Whelan J , Delamarter J F 1992 Murine endothelial leukocyte-adhesion molecule I is a close structural and functional homologue of the human protein.

This dual function is rather unique and is supported by two distinct sites in the molecule and two different receptors (Ikeda et a1 1993, Savage et a1 1992). The regulation of these processes depends on changes in the affinity of von Willebrand factor for GPIb, since soluble von Willebrand factor does not bind to the receptor unless an appropriate ‘modulator’ of the interaction is present. Two non-physiological modulators such as this have been used extensively-the antibiotic ristocetin (Howard & Firkin 1971, Scott et a1 1991) and the snake protein botrocetin (Read et a1 1978, Sugimoto et a1 1991).

The one homozygous animal died at 6 weeks of age and its remains were not available for analysis. 2 kb 5 I Domaln S I V VI VII TdCyt Structure of Disrupted Locus FIG. 3. Disruption of the murine vascular cell adhesion molecule (VCAM)-I gene. The structure of the murine VCAM-I gene is shown along with the VCAM-I knockout construct and structure of the targeted locus. Also shown are the location of restriction enzyme BglII sites used to identify mice carrying the mutated allele. A detailed description of the constructs and targeting in embryonic stem cells will be presented elsewhere.

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